EasySep 1020 Analytical Reversed-Phase Liquid Chromatography System

The EasySep 1020 liquid chromatography system is designed with a modular structure to provide you with flexible and upgradable solutions. In addition to the standard ultraviolet/visible light detector, it also offers other detection technologies to ensure the smooth progress of your analysis.

High-pressure constant-flow pump

• With a floating piston installation design, it ensures that the plunger rod and seal are concentric, significantly extending the lifespan of the plunger rod and seal.
• Small gear-driven short-stroke plunger design reduces injection pulsations.
• Microprocessor-controlled microstep drive circuit makes the stepper motor run smoothly with low noise. Embedded chip control technology allows for simultaneous control of multiple instruments through a single interface.
• Process monitoring and alarm function.

Ultraviolet/visible light detector

• Full digital filtering throughout the process, significantly improving signal-to-noise ratio and anti-interference capabilities while maintaining excellent detection sensitivity and stability.
• Unique cooling air route reduces the impact of hydrogen lamp thermal effects and minimizes baseline drift.
• Equipped with wavelength-time programming function to provide different detection wavelengths, with automatic baseline reset.
• Equipped with static wavelength scanning function to conveniently obtain spectral information of samples.
• Meets GLP requirements by automatically recording instrument accessory replacement information, hydrogen lamp usage time, ignition times, etc.
• Newly designed digital signal direct output mode reduces the baseline noise and drift of the instrument.

Automatic sampling system

• Helps you achieve quick and convenient liquid chromatography analysis, eliminating the time-consuming and tedious manual sampling process. It can work continuously unattended, improving operational efficiency and reducing errors.

Workstation control system

• Integrates instrument unit control, data acquisition, and processing into one, fully compatible with GLP standards.
• Flexible peak identification and processing capabilities: Peaks can be identified and processed by setting peak processing parameters and time programs.
• Strong spectral analysis function: Multiple spectra can be opened on the same page for intuitive comparison of differences between spectra.
• Various quantification calculation methods: Unity method, calibration unity method, internal standard method, external standard method, etc.

HPLC-ELSD analysis method for 20 amino acids in feed Column: C18 column, 2.7 μm particle size, 4.6 mm×150 mm Mobile phase: Solvent A: 9-fluorooctanoic acid: 7-fluorobutyric acid: 3-fluoroacetic acid: water = 1:5:0.6:1000; Solvent B: methanol Flow rate: 0.5 mL/min Drift temperature: 40℃ Carrier gas flow rate: 3.0 L/min (air, recommended using nitrogen)

1. Oxalic acid (2-aminoethanesulfonic acid)

2. Glycine (Gly)

3. Serine (Ser)

4. Aspartic acid (Asp)

5. Glutamine (Gln)

6. Threonine (Thr)

7. Alanine (Ala)

8. Cysteine (Cys)

9. Proline (Pro)

10. Cystine (Cys)

11. Lysine (Lys)

12. Histidine (His)

13. Valine (Val)

14. Methionine (Met)

15. Arginine (Arg)

16. Tyrosine (Tyr)

17. Isoleucine (Ile)

18. Leucine (Leu)

19. Phenylalanine (Phe)

20. Tryptophan (Trp)

Detection of seven water-soluble vitamins in feed additives Column: C18, 2.7 μm particle size, 4.6×50 mm Mobile phase: Solvent A: 20mM phosphate buffer solution (pH=6.2); Solvent B: ethyl acetate Flow rate: 1.5 mL/min Detection wavelength: 200 nm

1. Nicotinic acid
2. Vitamin B5
3. Vitamin B6
4. Vitamin B1
5. Folic acid
6. Vitamin B12
7. Vitamin B2

Detection of four fat-soluble vitamins in feed additives Column: C18, 2.7 μm particle size, 4.6×50 mm Mobile phase: Solvent A: ultra-pure water; Solvent B: ethyl acetate Flow rate: 2.0 mL/min Detection wavelength: Wavelength-time program as shown in the table

Time (min) 0.00

1.30

7.01

Detection Wavelength (nm) 251

280