Globalsil Preparative Columns
◆ High Purity and Excellent Chemical Stability: The packing material features high purity and superior chemical stability.
◆ High Resolution: Enables high-resolution and rapid separations.
◆ Increased Loading Capacity: Optimized to improve sample loading capacity.
◆ High Mechanical Strength: High mechanical strength ensures a long service life.
◆ Linear Scalability: Supports linear scale-up from analytical to preparative scales.
◆ Service Support: Technical services for the repacking and maintenance of preparative columns are available.
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1. Specifications
Preparative liquid chromatography columns are provided in a variety of specifications, and technical services for the repacking and maintenance of these columns are also available
Various specifications available for selection:
| Chromatographic Media | ||||
| Packing Material Type | Particle Size(um) | Pore size(A) | Surface Area(m/g) | Carbon Loading(%) |
| 120-10-C18-AP | 10 | 120 | 300 | 17 |
| 120-10-C18-BP | 10 | 120 | 300 | 15 |
| 120-10-C18-BIO | 10 | 120 | 300 | 20 |
| 120-10-C8-B10 | 10 | 120 | 300 | 12 |
| 200-10-C8-BI0 | 10 | 200 | 200 | 8 |
| 120-10-C4-BI0 | 10 | 120 | 300 | 9 |
| 300-10-C4-BIO | 10 | 300 | 100 | 3 |
| 120-10-NH: | 10 | 120 | 300 | 4 |
| 120-30/50-C18-AP | 40 | 120 | 300 | 17 |
| 120-30/50-C18-BP | 40 | 120 | 300 | 15 |
2. Application
Sample Name: SY-32
Preparation Objective: The target analyte content is 17% before purification, and >95% after purification.
The following are the chromatograms before preparation, during preparation, and after preparation:
Pre-purification Chromatogram (Target Purity 17%)
Column: 4.6 mm ID × 250 mm GS-120-5-AP
Mobile Phase:
- Solvent A: Acetonitrile with 0.1% Trifluoroacetic Acid (TFA)
- Solvent B: Water with 0.1% Trifluoroacetic Acid (TFA)
Gradient:
- 0.01 min: 12% A, 88% B
- 25.0 min: 37% A, 63% B
- 25.1 min: 100% A, 0% B
- 30.0 min: STOP
Detection Wavelength: UV @ 220 nm
Temperature: 25 °C
Injection Volume: 20 μL
Preparative Chromatogram
Column: 20 mm ID × 250 mm GS-120-10-C18-AP
Mobile Phase:
- Solvent A: Acetonitrile with 0.1% Trifluoroacetic Acid (TFA)
- Solvent B: Water with 0.1% Trifluoroacetic Acid (TFA)
Gradient:
- 0.01 min: 14% A, 86% B
- 25.0 min: 18% A, 82% B
- 25.1 min: 100% A, 0% B
- 30.0 min: STOP
Detection Wavelength: UV @ 220 nm
Temperature: 25 °C
Injection concentration: 11.5 mg/mL
Purified analytical chromatogram (target purity: 95.85%)
Column: 4.6 mm ID × 250 mm GS-120-5-C18-AP
Mobile Phase:
- Solvent A: Acetonitrile with 0.1% Trifluoroacetic Acid (TFA)
- Solvent B: Water with 0.1% Trifluoroacetic Acid (TFA)
Gradient:
- 0.01 min: 12% A, 88% B
- 25.0 min: 37% A, 63% B
- 25.1 min: 100% A, 0% B
- 30.0 min: STOP
Detection Wavelength: UV @ 220 nm
Temperature: 25 °C
Injection Volume: 20 μL
Other details:
- Transportation:By air if not specified.