Laser Induced Fluorescence Detector （ LIF Detector ）

• Introduction of TriSep™-2100LIF Detector
• Applications of LIF detector
• About Fluorescence and Laser Induced Fluorescence (LIF)

Introduction of TriSep™-2100LIF Detector

TriSep™ -2100LIF laser induced fluorescence detector manufactured by Unimicro Technologies, Inc. was first launched in 2004. With its patented designs in optical and mechanical structure, TriSep™ -2100LIF delivers an excellent performance in high sensitivity and versatility. Having designed in modular, TriSep™ -2100LIF is user-friendly.

• Sensitivity
  
  Using a laser beam as the emission light source, TriSep ™ -2100LIF offers excellent performance in sensitivity with its unique optical designs. For example, as little as 10 ^-12M FITC can be easily detected with a signal-to-noise ratio above 10 (100μm ID capillary flowcell, 473nm DPSS laser at 10mW).
  
  
• Versatility
  
  TriSep™-2100LIF provides a new choice for analytical chemists and biologists interested in trace analysis that requires high sensitivity. With an elegant design of mechanical structure, TriSep™-2100LIF can be coupled with CE, CEC, Capillary-LC, Micro-HPLC, and HPLC.
  
• Application fields
  
  Pharmacology, Neurochemistry, molecular biology, biochemistry, immunology, toxicology, environmental analysis, cosmetology, oncology, food, forensic, and etc.
  
  
• Specifications

Applications of LIF detector

• Sensitivity
• Separation of amino acids by CE, detected by LIF
• Separation of 5 aliphatic amine-FITC derivatives by pCEC, detected by LIF

Sensitivity

Concentration: FITC 1.0×10-12mol/l Detector: Laser Induced Fluorescence Detector (LIF) Excitation wav. : 473nm Emission wav. : 520nm

Separation of amino acids by CE, detected by LIF

Capillary column：100μm i.d.×58cm
Buffer：10mM borate buffer (pH9.3) Voltage：10kV
Detector：LIF，excitation wavelength:473 nm, emission wavelength: 520 nm
Injection：10kV,2S Sample：7×10^-9M Amino Acid, FITC dyed

Separation of 5 aliphatic amine-FITC derivatives by pCEC, detected by LIF

Column: EP-100-20-3-C18 Solvent: (A) 64% (v/v) CH3CN/36% 4mM Na2HPO4 (pH=8) (B) 20% (v/v) CH3CN/80% 4mM Na2HPO4 (pH=8) Conditions: 0 – 7 min 0% A. 7 – 20min 0% - 100 A Pressure: 6.4 Mpa Power Voltage: 1kV Detector: LIF detector Emission: 473 nm Excitation: 520 nm Injection: 20nL Sample: 1. 3-methyl-isobutylamine 2. n-pentylamine 3. n-hexylamine 4. n-heptylamine 5. n-octylamine

About Fluorescence and Laser Induced Fluorescence (LIF) detection

Fluorescence is the phenomenon in which absorption of light of a given wavelength by a fluorescent molecule is followed by the emission of light at longer wavelengths. The distribution of wavelength-dependent intensity that causes fluorescence is known as the fluorescence excitation spectrum, and the distribution of wavelength-dependent intensity of emitted energy is known as the fluorescence emission spectrum.

Fluorescence detection has three major advantages over other light-based investigation methods: high sensitivity, high speed, and safety. The point of safety refers to the fact that samples are not affected or destroyed in the process, and no hazardous byproducts are generated.

Sensitivity is an important issue because the fluorescence signal is proportional to the concentration of the substance being investigated. Whereas absorbance measurements can reliably determine concentrations only as low as several tenths of a micromolar, fluorescence techniques can accurately measure concentrations one million times smaller – at pico- and even femtomolar level. Quantities less than an attomole (<10 ^-18 mole) may even be detected.

Laser Induced Fluorescence detection technologies, using a laser beam to excite the fluorescent molecules, make unsurpassed sensitivity in chemical and biochemical assays. For example, with TriSep ™ -2100LIF Laser Induced Fluorescence Detector, as little as 10 ^-12 M FITC can be easily detected with a signal-to-noise ratio above 10 (100μm ID capillary flowcell, 473nm DPSS laser at 10mW).