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UV/Vis on-column detector
Overview of TriSep ™ –2100 UV/Vis On-column Detector
 Unimicro's TriSep ™ UV/Vis detector is made for on-column detection technologies, specially for pressurized Capillary Electro-Chromatography (pCEC) systems, Capillary Electrophoresis (CE) system and micro-HPLC. It is a high-performance and multi-functional ultra-violet visible spectrophotometric on-column detector, incorporating both deuterium and tungsten halogen lamps. Each unit has 3 measurement modes - single wavelength, dual wavelength and wavelength scanning.
Features of TriSep™ –2100 UV/Vis On-column Detector
- Excellent S/N ratio performance
An improved optical system and a high-order digital filter have enhanced the unit's Signal to Noise ratio. Enhanced wavelength accuracy, reproducibility, and broader wavelength range measurement have heightened the unit's basic performance.
- Advanced functions
The dual wavelength mode provides chromatograms or ratio chromatograms of two wavelengths simultaneously, and the wavelength-scanning mode provides absorbance spectra. There is also a full set of Time Program functions.
- No optical alignment needed when lamps are replaced
Adjustment of the optical alignment is not required when the lamps are replaced. The deuterium lamp has a longer life than
previous models. And the unit records the total time that the lamp has been used. 
Specifications of TriSep™ –2100 UV/Vis On-column Detector
Item |
TriSep™—2100 detector |
Light source |
Deuterium lamp |
Wavelength range |
190-600nm Cut-off filter to eliminate second order diffraction is automatically activated for the 371—600nm range. |
Spectral bandwidth |
8nm |
Wavelength accuracy |
1nm |
Wavelength reproducibility |
0.1nm |
Drift |
5×10-4 AU/hour Max. |
Noise Level |
6×10-6 AU |
Dual wavelength mode |
Measurement Wavelength |
Selectable, two wavelengths in 190—370nm or 371—600nm range |
Sampling frequency |
1.2 sec for one wavelength |
Response |
Selectable in 10 steps corresponding to time constant 0.05, 0.1, 0.5, 1.0, 1.5, 2.0, 3.0, 6.0, 8.0, 10.0 seconds |
Range |
Can be set between 0.0001 and 2.56 AUFS in 0.0001 AUFS steps |
Zero adjustment |
Auto zero function, baseline shift function |
Polarity switching Wavelength scanning function |
Possible Performs wavelength scanning with flow stopped. Data stored in three files, one of which stores background. Background is subtracted from sample scans. File data is not saved up (lost when power is turned OFF). |
Wavelength steps |
1-5nm, selectable in 5 steps, |
Scanning speed |
2-5nm when W lamp is used. 10-50nm/sec, settable in 5 steps (according to the wavelength step) |
Spectrum plot output speed |
1, 3, 10 nm/sec |
Ratio chromatogram |
Outputs the absorption ratio of two wavelengths |
Time Program |
Available in detector or by system controller |
|
Set items |
Wavelength (including dual wavelengths), auto zero, range, marker, response, wavelength scanning, event, polarity, lamp ON/OFF, loop |
Number of steps (detector program) |
Maximum 32 steps |
Output |
Output for recorder |
10mV recorder terminals |
Output for integrator |
6 steps: 0.5, 1, 2, 4, 1.25 and 2.5 AU/V Integrator output terminals output chromatogram in single wavelength mode or the Ch1 chromatogram in dual wavelength mode. Ratio chromatogram, spectrum, and Ch2 chromatogram are output from the recorder output terminals. |
Lamp hour-meter |
Records up to 9999.9 hours |
Size |
260(W) 140(H) 430(D) mm |
Weight |
13 kg |
Operating temperature range |
4°C — 35°C |
Power supply |
110-120V AC, 10 % 150VA 50-60Hz
220-230V AC/240V AC, 10 % 150VA 50-60Hz |
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