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Home > Products > Columns > Capillary Columns
Capillary Columns
Introduction
 ElectroPak™ capillary columns are packed columns, which can be easily coupled with CEC system, capillary HPLC system or capillary LC-MS system.
Patented electro-packing technologies make ElectroPak™ capillary columns high efficiency, high resolution and high speed . These advantages will bring you more findings and new experiences in your analytical work.
The capillary columns are packed with various categories packing materials, such as Si, C18, C8, Phenyl, CN, Diol, Chiral materials, and so on. And several particle and pore sizes can be offered. They are available in diversified inside diameters, outside diameters and lengths.
High efficiency separation(500,000 plates/m) of 14 explosives in 7 min. See ref. Bailey, C. & Yan, C., Anal. Chem ., 1998 , 70, 3275
Capillary Electrochromatography Start-Up Kits
Our CEC-Kit includes a manual syringe pump and a capillary inspector, everything needed to transform a CE instrument into a CEC system that provides HPLC-type of separation with high efficiency, high resolution and high selectivity. The manual syringe pump and the capillary inspector can be used to condition and inspect a packed capillary column conveniently prior to its installation into the CE or CEC instrument.
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Capillary Inspector
CEC-CI-001 |
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Manual Syringe Pump
(CEC-MSP-001) |
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CEC KIT
| Product No. |
Description |
Price |
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| CEC-SU-KIT-001 |
A manual syringe pump and a capillary inspector (including CEC-MSP-001 and CEC-CI-001) |
$99.99 |
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| CEC-MSP-001 |
A manual syringe pump plus an extra 0.5-mL syringe and extra 365- m m i.d. polyethylene tubing |
$46.990 |
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| CEC-CI-001 |
A capillary inspector including a 30x microscope and a light source plus an extra bulb and three "AA" batteries |
$53.99 |
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Fused Silica Capillaries
| Product No. |
Description |
Price |
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| FSC-075-365 |
5 meter x 75um i.d. x 365- um o.d. FS capillary |
$40 |
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| FSC-100-365 |
5 meter x 100um i.d. x 365- um o.d. FS capillary |
$40 |
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| FSC-100-365 |
5 meter x 150 um i.d. x 365- um o.d. FS capillary |
$40 |
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Polyethylene Tubing
| Product No. |
Description |
Price |
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| PET-365 |
1 meter x 365- um i.d. x 1090- um o.d. polyethylene tubing |
$15 |
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Pre-Installed Columns into Cartridges
| Product No. |
Description |
Price |
PIS-200-AP
PIS-800-AP |
Pre-installed CEC column in Beckman's P/ACE cartridge with 200- um or 800- um aperture. Send us your cartridge after receiving this package. |
Free
Free |
Free Installation of CEC Column
| Product No. |
Description |
Price |
| FI-Beckman |
Free installation of Electropak TM column into customer supplied |
Free |
| FI-HP |
CE cartridge or cassette from most CE manufacturers including |
Free |
| FI-Bio-Rad |
Beckman, HP, Bio-Rad, Thermo Separation Products, etc. |
Free |
| FI-TSP |
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Free |
Instruction for Conditioning of Capillary Columns for (CEC)
- Mobile Phase
a) Start with a similar mobile phase as one would use in HPLC for a particular separation. Typical mobile phase in CEC is, for example, 80% CH3CN mixed with 1-10mM buffer, such as TRIS, MES, or Na2B4O7. In order to generate enough electroosmotic flow (EOF) the pH should be between 3 and 9 for a column packed with silica based materials (e.g. ODS).
b) The mobile phase should be filtered and thoroughly degassed with ultra sonication plus vacuum for ~10 minutes.
- Installation of ELECTROPAKTM Column
a) Remove the window protection sleeve and the end caps (Teflon tubing ) from the column.
b) Install the ELECTROPAKTM column in the CE cartridge in the same way as with any other capillary columns.
- Conditioning of ELECTROPAKTM Column
a) Fill the Manual Syringe Pump (Product No.: CEC-MSP-001) slowly with the mobile phase. Make sure that there are no bubbles in the syringe and in the attached Teflon tubing.
b) Carefully insert the inlet of the ELECTROPAKTM column (~3mm) into the Teflon tubing connected to the syringe.
c) Condition the column with the Manual Syringe Pump by tightening the handle just enough to generate a moderate pressure (~500 psi). The pressure can last for ~30 minutes once the handle is tightened.
d) Watch the bubbles marching out of the capillary (~0.2 mm/s) using our Capillary Inspector (Product No.: CEC-CI-001) at the window or after the window (open section of the column). It may take 2 hours to get the column completely conditioned. Slowly but surely! Do not expect any droplet from the outlet since the evaporation rate is usually faster than the flow rate.
e) Wait for 10 minutes after the bubbles (if any) in the column finally disappear . Release the pressure by loosening the Manual Syringe Pump. Let the pressure dissipate for ~5 minutes.
f) Cut off extra length of the outlet capillary if necessary (never cut the inlet side of the column!). Disconnect the column from the syringe and install the cartridge in the CE system immediately.
g) Condition the column in the CE system at a relatively low voltage (less than 5 kV) for approximately 15 minutes. Then, increase the voltage to the desired value. A typical current in a 75 µm i.d. x 25 cm column packed with 3 µm ODS with 80% CH3CN/4 mM Na2B4O7 is about 2µA at 20 kV.
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