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BiSep™-1100 High Performance Liquid Chromatography System

BiSep™-1100 High Performance Liquid Chromatography System (HPLC), manufactured by Unimicro Technologies, Inc., is a newly released separation system with good quality, reliability and accuracy.

BiSep™-1100 HPLC System is composed of detector and pumps. With modular design, the system is easily converted to microbore HPLC, which can save 75% of solvent and sample.

BiSep™-1100 Detector

Overview

The Unimicro Technologies BiSep detector is high-performance, multi-function system ultra-violet with a visible spectrophotometric detector, for use in high performance liquid chromatography. The BiSep detector uses a deuterium lamp as its light source and is intended primarily for analyses in the ultraviolet region. The BiSep detector incorporates both deuterium and tungsten halogen lamps. The deuterium lamp is used for ultraviolet applications, as in the BiSep detector, while the tungsten lamp extends the analytical capability into the visible region. Each unit has 3 measurement modes - single wavelength, dual wavelength and wavelength scanning. The dual wavelength mode performs simultaneous detection of two wavelengths, and can provide chromatograms of both the wavelengths, or a chromatogram of one wavelength and one ratio chromatogram. In wavelength scanning mode, the absorbance spectrum is measured. The wavelength scanning mode is designed to be used while the flow is stopped.

Features

Excellent S/N ratio performance

An improved optical system and a high-order digital filter have enhanced the unit's Signal to Noise ratio. Enhanced wavelength accuracy, reproducibility, and broader wavelength range measurement have heightened the unit's basic performance.
Advanced functions

The dual wavelength mode provides chromatograms or ratio chromatograms of two wavelengths simultaneously, and the wavelength scanning mode provides absorbance spectra. There is also a full set of Time Program functions.
No optical alignment needed when lamps are replaced

No adjustment of optical alignment is required when the lamps are replaced. The deuterium lamp has a longer life than previously, and the unit records the total time the lamp has been used.

Specifications

Item		BiSep ™ —1100 detector
Light source		Deuterium lamp
Wavelength range		190-600nm Cut-off filter to eliminate second order diffraction is automatically activated for the 371—600nm range.
Spectral bandwidth		8nm
Wavelength accuracy		1nm
Wavelength reproducibility		0.1nm (*1)
Drift		2×10^-4 AU/hour Max. (250nm, room temperature constant, air in the cell) 3-10^-4 AU/hour Max. (250 nm, room temperature fluctuation less than 2°C, air in the cell)
Noise Level		0.35×10^-5 AU
Cell path length		8 µL
Cell pressure tolerance		12MPa {120kgf/cm² }
Cell materials in contact with liquid		SUS316, teflon, quartz
Cell inlet, outlet tubing diameter		SUS316 tubing 1/ 16” OD 0.3mmID
Dual wavelength mode (*3)	Measurement Wavelength	Selectable, two wavelengths in 190—370nm or 371—600nm range
	Sampling frequency	1.2 sec for one wavelength
Response		Selectable in 10 steps corresponding to time constant 0.05, 0.1, 0.5, 1.0, 1.5, 2.0, 3.0, 6.0, 8.0, 10.0 seconds
Range		Can be set between 0.0001 and 2.56 AUFS in 0.0001 AUFS steps

*1 Reproducibility when the wavelength is changed after turning the power ON in single wavelength mode.
*2 In single wavelength mode.
*3 Noise level in dual wavelength mode varies depending on the wavelength. It may be necessary to use less sensitive detector settings (higher Range values) when performing dual wavelength analysis.

Item		BiSep ™ -1100 detector
Zero adjustment		Auto zero function, baseline shift function
Polarity switching Wavelength scanning function		Possible Performs wavelength scanning with flow stopped. Data stored in three files, one of which stores background. Background is subtracted from sample scans. File data is not saved up (lost when power is turned OFF).
	Wavelength steps	1-5nm, selectable in 5 steps, 2-5nm when W lamp is used. 10-50nm/sec, settable in 5 steps (according to the wavelength step) 1, 3, 10 nm/sec
	Scanning speed
	Spectrum plot output speed
Ratio chromatogram		Outputs the absorption ratio of two wavelengths
Time Program		Available in detector or by system controller
	Set items	Wavelength (including dual wavelengths), auto zero, range, marker, response, wavelength scanning, event, polarity, lamp ON/OFF, loop
	Number of steps (detector program)	Maximum 32 steps
Output	Output for recorder	10mV recorder terminals
	Output for integrator	6 steps: 0.5, 1, 2, 4, 1.25 and 2.5 AU/V Integrator output terminals output chromatogram in single wavelength mode or the Ch1 chromatogram in dual wavelength mode. Ratio chromatogram, spectrum, and Ch2 chromatogram are output from the recorder output terminals.
Lamp hour-meter		Records up to 9999.9 hours
Size		260(W) 140(H) 430(D) mm
Weight		13 kg
Operating temperature range		4°C — 35°C
Power supply		110-120V AC, 10 % 150VA 50-60Hz 220-230V AC/240V AC, 10 % 150VA 50-60Hz

BiSep™-1100 Pump

Overview

The pump is a dual reciprocating plunger solvent delivery module. It offers improved accuracy and sensitivity in the BiSep™-1100system. In addition to the delivery module, the BiSep™-1100 system also requires, at a minimum, an injector, column, detector, high voltage module and system controller. Note that each of these is purchased separately from the pumping unit. Please consult our neareat branch/sales office or agent regarding the components that are necessary for your system.

Features

High Gradient Accuracy and Ultra-Low Flow Pulsation

Both the duration and the magnitude of the pump’s flow pulsation are significantly reduced in comparison to conventional pumps. This is enabled by a design which drives the plunger at high speed and reduces discharge to a micro volume: 10µl per plunger stroke. This lack of flow pulsationcapacity to a micro column 10 allows detectors such as differential refractometers and electrochemical detectors to be used in their high sensitivity range, an application impossible with conventional pumps. The solvent delivery system also provides highly accurate gradent delivery in a semi-micro volume range.
A Range of Gradient Delivery Functions

The pump can be used in both low- and high- pressure gradient delivery modes. The high-pressure gradient delivery mode is highly accurate with minimum time lag.
Automatic Plunger Seal Rinsing

An optional automatic mechanism rinses the back of the plunger seal, precenting premature
seal failure due to crystallization of high-salt-concentration buffer solutions.

Specifications

The system specifications

Item		Specification
Pump type		Micro- volum double plunger pump(approx. 10 µ L per stroke )
Pumping methods		Constant flow delivery and constant pressure delivery
Constant flow pumping	Flow rate setting range	1.0-39.2MPa (10-400kgf/cm² )0.001-5mL/min[1.0-39.2MPa(10-400kgf/cm² )] 1.0-19.6MPa(10-200kgf/cm² )5.001-9.999mL/min[1.0-19.6MPa(10-200kgf/cm² )]
	Flow rate accuracy	± 2% or ± 2 µ L/min, whichever is greater(when water is pumped at 0.01-5mL/min, 1.0-39.2MPa(10-400kgf/cm² ), with room temperature 20°C) A different accuracy applies to low-pressure gradient delivery
	Flow rate precision	Within ± 0.3% (when water is pumped at 0.1-5mL/min, 1.0-39.2MPa(10-400kgf/cm² ), with room temperature 20°C) )
Constant pressure pumping	Pressure setting range	1.0-39.2MPa(10-400kgf/cm²)[0.1MPa (1kgf/cm² )steps]
	Pressure accuracy	± 10% or1.0MPa (10kgf/cm² ), whichever is greater
	Pressure limits	Upper and lower limits
Damper		Interior capacity 400 µ L approx
Liquid-contacting part materials		SUS316, ruby, sapphire, Teflon ®, HastelloyC®, PEEK, perfluoroelastomer Zirconia, PCTFE
Suction filter		10 µ m mesh
Line filter		5 µ m mesh, capacity 30 µ L approx.
Time program		Commands for flow rate, pressure, EVENT functions, LOOP (for program repetition ) 10files,320 steps.
Pressure display accuracy		**** ± 2% or 0.5MPa (5kgf/cm² ), whichever is greater
Plunger cleaning line		Automatic rinsing possible with optional kit
Size		W260 × H 140 × D 420mm , excluding projections
Weight		11kg
Operating temperature range		4-35°C

High-pressure gradient system specifications

Item	Specification
Number of solvents mixed	2
Gradient profile	Step and linear possible at multiple levels
Maximum program steps	Max.320 steps per program. 20program files
Program duration	Max.999.99 minutes (in 0.01minute steps
Mix ratio setting range	0-100% (in 0.1% steps)
Concentration accuracy	± 1% [with aqueous acetone/water, at 0-100%, 0.1-3mL/min,1.0-39.2MPa(10-400kgf/cm²)]
Flow rates possible	0.001-9.999mL/min