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Pressurized Capillary Eletrochromatography (pCEC)

Instrument

Features of TriSep™-2100 pCEC system
Overview of TriSep™-2100 pCEC system
Main Specifications of TriSep™-2100 pCEC system
Capillary Electrochromatography Start-Up Kits

Features of TriSep™-2100 pCEC system

Ultra Performance in Separation

Ultra Efficiency: Over 250,000 plates/m, 4 times better than HPLC
Ultra Speed: 5.5 mm/s of linear velocity, 5 times faster than HPLC
Ultra Peak Capacity: 3 times better than HPLC
Ultra Reproducibility: Excellent reproducibility like HPLC, and much better than CE
All in One

Modular design and flexible combination make it possible to run CE, micro-HPLC and pCEC on the same TriSep™-2100 system!·
Advanced Mechanism

User of TriSep™-2100 will readily enjoy the leisure and excitement in research brought by the system features of high efficiency, high selectivity, high resolution and fast speed. ·
Method Development

The direct transplanting of method from classic HPLC separation condition greatly shortens the time needed for method development.·
Gradient Eluting CEC

Elaborate micro-fluid control module dramatically decreases system dead-volume. High-pressure gradient eluting CEC mode dramatically improves the separation power. ·
Dedicated Software

User-friendly TriSep™ Workstation software, which fully complies with GLP regulations, facilitates powerful data analysis!

Overview of TriSep™-2100 pCEC system

Pressurized capillary electrochromatography (pCEC) is a new revolutionary separation technology which achieves ultra performance. In pCEC, the retention mechanism is based on both chromatographic partition and electrophoresis. Thanks to the contribution of the dual mechanisms, pCEC successfully makes use of columns with small particles of 1.5µm, and dramatically enhances the efficiency, speed, peak capacity, reproducibility and sensitivity, compared to traditional HPLC and CE. Combined with on-column detection techniques, pCEC could be readily coupled with UV, LIF, and MS. Multi-mechanism makes CEC an ideal technique for the analysis of complicated samples in biological and chemical fields.

Currently, CEC technology is widely applied in various fields, including Pharmaceutical, Life Science, Toxicology, Forensic Medicine, Health Products, Chemical, Petrochemical, Environment, Chiral Compound, Food/Beverage, etc.

As the leader in nano and micro separations, Unimicro Technologies presented the world’s first dedicated pCEC system TriSep™-2000 and then developed the second generation pCEC system, TriSep™-2010GV. In 2004, Unimicro Technologies came up with the latest version, TriSep™-2100. Revolutionary design and engineering dramatically elevate the system performance, compared to the former models. Powerful technical support on application, brand-new coupling interface with various up-to-date detection technologies and patented EletroPak™ capillary columns make TriSep™-2100 an ideal tool for explorers in all research fields mentioned above.

Main Specifications of TriSep™-2100 pCEC system

Solvent Delivery System

Dual reciprocating plunger solvent delivery module, high gradient accuracy and ultra-low flow pulsation, automatic plunger seal rinsing.

Item		Specification
Pump type		Micro- volume double plunger pump (approx. 10 m L per stroke )
Pumping methods		Constant flow delivery and constant pressure delivery
Constant flow pumping	Flow rate setting range	0.001-5mL/min (1.0-39.2MPa), 5.001-9.999mL/min(1.0-19.6MPa)
	Flow rate accuracy	± 2% or ± 2 m L/min, whichever is greater(when water is pumped at 0.01-5mL/min, 1.0-39.2MPa, with room temperature 20 °C )
	Flow rate precision	Within ± 0.3% (when water is pumped at 0.1-5mL/min, 1.0-39.2MPa, with room temperature 20 °C )
Constant pressure pumping	Pressure setting range	1.0-39.2MPa [0.1MPa steps]
	Pressure accuracy	± 10% or1.0MPa, whichever is greater
	Pressure limits	Upper and lower limits
Time program		Commands for flow rate, pressure, EVENT functions, LOOP (for program repetition) 10files, 320 steps.
Pressure display accuracy		± 2% or 0.5MPa, whichever is greater
Plunger cleaning line		Automatic rinsing possible with optional kit
Size		W260 × H 140 × D 420mm , excluding projections
Weight		11kg
Operating temperature range		4-35 °C

High-pressure Gradient System Specifications

Item	Specification
Number of solvents mixed	2
Gradient profile	Step and linear possible at multiple levels
Maximum program steps	Max.320 steps per program. 20program files
Program duration	Max.999.99 minutes (in 0.01minute steps)
Mix ratio setting range	0-100% (in 0.1% steps)
Concentration accuracy	± 1% (with aqueous acetone/water, at 0-100%, 0.1-3mL/min,1.0-39.2MPa)
Flow rates poss inlet	0.001-9.999mL/min

Nano Injection Valve:
Optional sample loop volumes: 10 nl, 20 nl, 50 nl

UV/Vis Detector

Item			TriSep ™ —2100 detector
Light source			Deuterium lamp
Wavelength range			190-600nm Cut-off filter to eliminate second order diffraction is automatically activated for the 371—600nm range.
Spectral bandwidth			8nm
Wavelength accuracy			1nm
Wavelength reproducibility			0.1nm
Drift			5 ×10 ^-4 AU/hour Max.
Noise Level			6 × 10 ^-6 AU
Dual wavelength mode		Measurement Wavelength	Selectable, two wavelengths in 190—370nm or 371—600nm range
		Sampling frequency	1.2 sec for one wavelength
Response			Selectable in 10 steps corresponding to time constant 0.05, 0.1, 0.5, 1.0, 1.5, 2.0, 3.0, 6.0, 8.0, 10.0 seconds
Range			Can be set between 0.0001 and 2.56 AUFS in 0.0001 AUFS steps
Zero adjustment			Auto zero function, baseline shift function
Polarity switching Wavelength scanning function			Possible Performs wavelength scanning with flow stopped. Data stored in three files, one of which stores background. Background is subtracted from sample scans. File data is not saved up (lost when power is turned OFF).
Wavelength steps			1-5nm, selectable in 5 steps,
Scanning speed			2-5nm when W lamp is used. 10-50nm/sec, settable in 5 steps (according to the wavelength step)
Spectrum plot output speed			1, 3, 10 nm/sec
Ratio chromatogram			Outputs the absorption ratio of two wavelengths
Time Program			Available in detector or by system controller
	Set items		Wavelength (including dual wavelengths), auto zero, range, marker, response, wavelength scanning, event, polarity, lamp ON/OFF, loop
	Number of steps (detector program)		Maximum 32 steps
Output	Output for recorder		10mV recorder terminals
	Output for integrator		6 steps: 0.5, 1, 2, 4, 1.25 and 2.5 AU/V Integrator output terminals output chromatogram in single wavelength mode or the Ch1 chromatogram in dual wavelength mode. Ratio chromatogram, spectrum, and Ch2 chromatogram are output from the recorder output terminals.
Lamp hour-meter			Records up to 9999.9 hours
Size			260(W) 140(H) 430(D) mm
Weight			13 kg
Operating temperature range			4°C — 35°C
Power supply			110-120V AC, 10 % 150VA 50-60Hz 220-230V AC/240V AC, 10 % 150VA 50-60Hz

High Voltage power Supply

Output voltage	0 ~ ± 30 kV
Output current:	0 ~ 100 µA
Max Output power	4 W
Net weight	12 kg

LIF Detector (Optional)

Relative Fluorescence Units (RFU) range	0 to 1000 RFU
Dynamic Range (at RFU range 1000)	10 ⁶
Sensitivity	1×10 ^-11 M with S/N>2
Noise	<0.005 RFU
Drift	<0.01 RFU
Wavelength Range (for optics)
Excitation	300 to 700 nm
Emission	350 to 750 nm
Software compatibility	TriSep-LIF software
Laser	10 mW 473 nm DPSS Laser
Power Requirements	AC110/220 V, 50/60 Hz
Temperature Operating Range	15-35 ° C

Capillary Electrochromatography Start-Up Kits

Our CEC-Kit includes a manual syringe pump and a capillary inspector, everything needed to transform a CE instrument into a CEC system that provides HPLC-type of separation with high efficiency, high resolution and high selectivity. The manual syringe pump and the capillary inspector can be used to condition and inspect a packed capillary column conveniently prior to its installation into the CE or CEC instrument.

Capillary Inspector

CEC-CI-001

Manual Syringe Pump

(CEC-MSP-001)

Product No.	Description	Price
CEC-SU-KIT-001	A manual syringe pump and a capillary inspector (including CEC-MSP-001 and CEC-CI-001)	$99.99
CEC-MSP-001	A manual syringe pump plus an extra 0.5-mL syringe and extra 365- µ m i.d. polyethylene tubing	$46.99
CEC-CI-001	A capillary inspector including a 30x microscope and a light source plus an extra bulb and three "AA" batteries	$53.99

Fused Silica Capillaries

Product No.	Description	Price
FSC-075-365	5 meter x 75- µm i.d. x 365- µm o.d. FS capillary	$40
FSC-100-365	5 meter x 100- µm i.d. x 365- µm o.d. FS capillary	$40

Polyethylene Tubing

Product No.	Description	Price
PET-365	1 meter x 365-µm i.d. x 1090- µm o.d. polyethylene tubing	$15

Pre-Installed Columns into Cartridges

Product No.	Description	Price
PIS-200-AP PIS-800-AP	Pre-installed CEC column in Beckman's P/ACE cartridge with 200- µm or 800- µm aperture. Send us your cartridge after receiving this package.	Free Free

Free Installation of CEC Column

Product No.	Description	Price
FI-Beckman	Free installation of Electropak TM column into customer supplied	Free
FI-HP	CE cartridge or cassette from most CE manufacturers including	Free
FI-Bio-Rad	Beckman, HP, Bio-Rad, Thermo Separation Products, etc.	Free
FI-TSP		Free

Instruction for Conditioning of Capillary Columns for (CEC)

Mobile Phase
a) Start with a similar mobile phase as one would use in HPLC for a particular separation. Typical mobile phase in CEC is, for example, 80% CH3CN mixed with 1-10mM buffer, such as TRIS, MES, or Na2B4O7. In order to generate enough electroosmotic flow (EOF) the pH should be between 3 and 9 for a column packed with silica based materials (e.g. ODS).
b) The mobile phase should be filtered and thoroughly degassed with ultra sonication plus vaccum for ~10 minutes.
Installation of ELECTROPAKTM Column
a) Remove the window protection sleeve and the end caps (Teflon tubing ) from the column.
b) Install the ELECTROPAKTM column in the CE cartridge in the same way as with any other capillary columns.
Conditioning of ELECTROPAKTM Column
a) Fill the Manual Syringe Pump (Product No.: CEC-MSP-001) slowly with the mobile phase. Make sure that there are no bubbles in the syringe and in the attached Teflon tubing.
b) Carefully insert the inlet of the ELECTROPAKTM column (~3mm) into the Teflon tubing connected to the syringe.
c) Condition the column with the Manual Syringe Pump by tightening the handle just enough to generate a moderate pressure (~500 psi). The pressure can last for ~30 minutes once the handle is tightened.
d) Watch the bubbles marching out of the capillary (~0.2 mm/s) using our Capillary Inspector (Product No.: CEC-CI-001) at the window or after the window (open section of the column). It may take 2 hours to get the column completely conditioned. Slowly but surely! Do not expect any droplet from the outlet since the evaporation rate is usually faster than the flow rate.
e) Wait for 10 minutes after the bubbles (if any) in the column finally disappear . Release the pressure by loosening the Manual Syringe Pump. Let the pressure dissipate for ~5 minutes.
f) Cut off extra length of the outlet capillary if necessary (never cut the inlet side of the column!). Disconnect the column from the syringe and install the cartridge in the CE system immediately.
g) Condition the column in the CE system at a relatively low voltage (less than 5 kV) for approximately 15 minutes. Then, increase the voltage to the desired value. A typical current in a 75 µm i.d. x 25 cm column packed with 3 µm ODS with 80% CH3CN/4 mM Na2B4O7 is about 2µA at 20 kV.