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Capillary Eletrochromatography (CEC)
CEC Applications
. HIGH RESOLUTION AND RAPID CEC ON 1.5μm NON-POROUS ODs
Fast separation on 1.5μm Non-porous ODS
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Figure 26. Fast separation of 16 EPA priority pollutants. Column: EP-100-20-1.5-C18 (1.5mm non-porous ODS, Micra Scientific, Inc., Northbrook, IL). Mobile phase: 70% CH 3 CN in 30% 2mM TRIS. Voltage: 55kV. Injection: 5kV/2s. Detection: LIF, ex: 257nm, em: 400nm. |
Electric Field Effect on Separation Speed
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Figure 27. Electrochromatograms showing the CEC separation of 5 PAHs using 1.5mm non-porous ODS particles. Column: EP-100-6.5-1.5-C18. Mobile phase: 70% CH3CN in 30% 2mM TRIS. Voltage: varied from 5 to 25kV. Injection: 5kV/2s. Detection: LIF, ex: 257nm, em: 400nm. |
High Resolution Separation on 1.5μm Non-porous ODS
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Figure 28. Baseline CEC separation of 16 PAHs on 1.5mm non-porous ODS. Column: EP-100-20-1.5-C18. Mobile phase: 65% CH 3 CN in 35% 2mM TRIS. Voltage: 29kV. Injection: 5kV/2s. Detection: LIF, ex: 257nm. em: 400nm. |
Effect of Linear Velocity on Efficiency
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Figure 29. Effect of linear velocity (EOF) on the height equivalent to a theoretical plate (HETP) for three PAHs. Column: EP-100-6.5-1.5-C18. Mobile phase: 70% CH 3 CN in 30% 2mM TRIS. Injection: 5kV/2s. Detection: LIF, ex: 257nm, em: 400nm.
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APPLICATIONS
Separation of Chiral Compounds on Silica
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Figure 30. CEC separation of synephrine enanthiomers. Column: EP-75-20-3-SI (3mm silica). Mobile phase: 10 mM TRIS (pH adjusted to 3.12 using H3PO4) with 14 mM hydroxypropyl-b-cyclodextrin as a mobile phase additive. Voltage: 15kV. Injection: 1kV/1s. Detection: UV at 214nm. Temperature: 25EC. CE instrument: Beckman P/ACE 2000.
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Separation of Basic Compounds on Silica
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Figure 31. CEC separation of basic compounds on silica stationary phase. Column: EP-75-20-3-SI (3mm silica). Mobile phase: 80% CH 3 CN/20% 10mM TRIS (pH adjusted to 8.29 using HCl). Voltage: 20kV. Injection: 5kV/5s. Detection: UV at 214nm. Temperature: 25EC. CE instrument: Beckman P/ACE 2000. Sample: 1. aniline, 2. cocaine hydrochloride, 3. berberine hydrochloride, 4. thebaine, 5. jatrorrhizine hydrochloride, 6. ephedrine hydrochloride, and 7. codeine phosphate. |
Separation of 25 aromatic Compounds
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Figure 32. CEC separation of a mixture of nitrogen containing aromatics and PAHs. Column: EP-75-30-3-C18. Mobile phase: 70% CH 3 CN/30% 4mM sodium tetraborate. (pH 9). Voltage: 20kV. Injection: 5kV/5s. Detection: LIF, ex: 257nm, em: 400nm.
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Separation of 14 Explosives
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Figure 33. Rapid CEC separation of explosives on 1.5mm non-porous ODS (Micra Scientific, Inc.). Column: EP-75-25-1.5-C18. Mobile phase: 7.5% CH 3 OH/7.5% isopropanol/85% 10mM MES/5mM SDS. Voltage: 11kV. Injection: 2kV/1s. Detection: UV at 254nm. |
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