Epinephrine

Fig.: Separation of epinephrine compound.

Experimental conditions:
Instrument: Trisep-2010 GV CEC system; Column: 200 mm×100μm I.D. capillary packed with 3μm C18 ;
Mobile phase: 0.1% (V/V) phosphoric acid and 0.1% sodium dodecate in 40% (V/V) acetonitrile,
Applied voltage: -3 kV;
Pressure applied on column: 14 Mpa;
Injection volume: 2 nl;
Sample: citrus extract;
Detector wavelength: UV 280 nm.
Peaks: 1.epinephrine, 2 propalepinephrine