Cefradine
Experimental Section
Instruments and Reagent
Experiments were performed on a TriSepTM-2010 Capillary Electrochromatography system (Shanghai Unimicro Analytical Technologies Co., Ltd.) with a 0-30kV high voltage power supply, a high speed scanning UV detector, a solvent delivery system and HW system software.
Reagent: methanol(HPLC grade);deionized water;cefradine dispersible tablet
Conditions
Column:Capillary C18
Mobile phase:Methanol/water/3.86%NaAc/4%HAc = 1536/400/30/6
Back pressure:2000 Psi
Flow:0.2 ml/min
Wavelength:254 nm
Temperature:Room temperature
Voltage:-10 kV
Results

Figure 1 Capillary Electrochromatogram of cefradine,-10.0 kV/-1.0μA
1.cefradine
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