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Eemodin and Chrysophanol in Longdan Pill

Fig.: Separation of longdan pill

Experimental conditions:
Instrument: Trisep-2010 GV CEC system;
Column: 200 mm×100μm I.D. capillary packed with 3μm C18 ;
Mobile phase: 0.1% (V/V) phosphoric acid in 85% (V/V) methanol,
Applied voltage: -20 kV;
Pressure applied on column: 14 Mpa;
Injection volume: 2 nl; Sample: longdan pill;
Detector wavelength: UV 254 nm;
Peaks: 1.emodin 2. chrysophanol