Porcine Insulin

Fig.1 standard

Fig.2 sample

Fig.3 sample

Experimental conditions:
nstrument: Trisep-2010 GV CEC system; Column: 200 mm×100μm I.D. capillary packed with 3μm C18 ;
Mobile phase: PBS/ /Methanol/Acetonitrile=40/40/20(V/V/V),
Applied voltage: -5 kV ;
Pressure applied on column: 14 Mpa;
Injection volume: 2 nl;
Detector wavelength: UV 220 nm;
Fig.1 std.; Fig 2,3 sample