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BSA+Myo Tryptic Digest

Fig.: Separation of BSA+myo tryptic digests.

Experimental conditions:
Instrument: Trisep-2010 GV CEC system;
Column: 500 mm×100μm I.D. Capillary packed with 3μm C18;
Mobile phase: A, 0.1% (V/V) TFA in 2% (V/V) acetonitrile/water; B, 0.1% (V/V) TFA in 98% (V/V) acetonitrile/water;
Linear gradient: 0% to 100% B in 60 min;
Pressure applied on capillary column: 14.8 Mpa;
Estimated injection volume: 20 nl;
Detector wavelength: 214 nm.

Fig.2: Separation of BSA+myo tryptic digests.

Experimental conditions:
Instrument: Trisep-2010 GV CEC system;
Column: 500 mm×100μm I.D. Capillary packed with 3μm C18;
Mobile phase: A, 0.1% (V/V) TFA in 2% (V/V) acetonitrile/water; B, 0.1% (V/V) TFA in 98% (V/V) acetonitrile/water;
Linear gradient: 0% to 100% B in 60 min;
Pressure applied on capillary column: 14.8 Mpa;
Estimated injection volume: 20 nl;
Detector wavelength: 214 nm.
Applied voltage:-5kV.