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Comparison of separations of 5 peptides

1 Experimental Section
1.1 Instruments and Reagent
Experiments were performed on a TriSepTM-2010 Capillary Electrochromatography system (Shanghai Unimicro Analytical Technologies Co., Ltd.) with a 0-30 kV high voltage power supply, a high speed scanning UV detector, a solvent delivery system and HW system software.
Reagent：Methanol（HPLC grade）；deionized water；phosphoric acid；Sodium lauryl sulphate；active protein
1.2 Conditions
Column：Capillary C18
Mobile phase：
A: methanol: 0.1M NaH2PO4 solution = 10 : 90 , pH=2.7(H3PO4)
B: methanol
Gradient：
Flow 0.1 ml/min
Wavelength：21 0nm
Temperature：Room temperature
Back pressure：2000 Psi
2 Results

Figure 1 Capillary chromatogram of active protein
Isocratic 99%A in five minutes, then gradient from 99% to 20% A in twenty minutes.