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Soybean Isoflavone

Figure : Separation of soybean isoflavone.

Experimental conditions:
Instrument: TriSep-2010 GV CEC system with a 0-30 kV high voltage power supply, a high speed scanning UV detector, a solvent delivery system and HW system software;
Column: 200 mm×100μm I.D. capillary packed with 3μm C18;
Mobile phase: 50% (V/V) methanol;
applied voltage: -15.0 kV;
Injection volume: 20 nl;
Detector wavelength: 254 nm;
Peaks: 1. Daidzin, 2. Genistin , 3.Daidzein, 4.Genistein