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Rutin

Fig.1: Separation of rutin.

Experimental conditions:
Instrument: Trisep-2010 GV CEC system; Column: 200mm×100μm I.D. capillary packed with 3μm C18;
Mobile phase: 0.1% (V/V) phosphoric acid in 50% (V/V) methanol;
Applied voltage: -5 kV;
Pressure applied on column: 14 Mpa;
Injection volume: 2 nl;
Detector wavelength: UV 380 nm;
Peaks: 1. rutin