homeproductsapplicationssupportabout unimicro
Chromatogram
. CEC advantage
. Chemica l& Petrochemical
. Chiral compound
. Environment
. Food & Beverage
. Health products
. Life science
. Pharmaceutical
. Toxicology & Forensic medicine
Articles

Home > Applications > Application Chromatograms > Food & Beverage

Preservatives

Experimental Section
Instrument and Reagent
Instrument :TriSepTM-2010GV capillary electrochromatography
(Shanghai Unimicro Technologies co.,Ltd.) UV Detector, High Pump, HW Workstation.
Back-pressure Restrictor :2000 psi
Reagent :Acetonitrile (HPLC); Redistilled Water; Phosphate
Sample :Methyl4-hydroxybenzoate, Ethyl4-hydroxybenzoate, Propyl4-hydroxybenzoate
The method of solution confect :Take fitting samples, use mobile phase dissolve, before used, filtrated 0.22m filter
Chromatography Conditions
Capillary column:100 m ID packed with 3m ODS, overall length 32 cm, effective length 20 cm
Mobile Phase : Actonitrile/ Phosphate buffer (PH3.5) =35/65(V/V),
5 mM(after mixing)
Pump flow rate : 0.02 ml/min; When injecting, the pump flow rate is 0.2 ml/min
Back-presure :2000 psi
Detection :254 nm
Temperature:Room temperature


Figure 1 (0kv)
1. Methyl4-hydroxybenzoate 2. Ethyl4-hydroxybenzoate 3. Propyl4-hydroxybenzoate


Figure 2 (-10kv)
1. Methyl4-hydroxybenzoate 2. Ethyl4-hydroxybenzoate 3. Propyl4-hydroxybenzoate

Copyright 2002-2005 Unimicro Technologies. All Rights Reserved
 Last Updated: 2005-03-02