Coffee

Figure : Capillary Electrochromatogram of coffee，-5.0kV/-14.3μA

Experimental conditions:
Instrument: TriSep-2010 GV CEC system with a 0-30 kV high voltage power supply, a high speed scanning UV detector, a solvent delivery system and HW system software;
Column: 200 mm×100μm I.D. capillary packed with 3μm C18;
Mobile phase: 10 mM NaH2PO4 buffer in 12% (V/V) acetonitrile;
Applied voltage: -5.0 kV;
Injection volume: 20 nl;
Detector wavelength: 254 nm;
Peaks: 1. caffeine