DL-Alanine

Fig.: Separation of DL-Alanine.

Experimental conditions:
Instrument: Trisep-2010 GV CEC system;
Column: 200 mm×100μm I.D. capillary packed with 3μm C18;
Mobile phase: 10 mM ammonium acetate buffer (pH 5.0) (with 2 mM htmlartame and 1mM copper ion);
Applied voltage: -10 kV;
Pressure applied on column: 14 Mpa;
Injection volume: 20 nl;
Detector wavelength: UV 214 nm