Pressurized Capillary Electrochromatography
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Product name:TriSep®3000 pCEC
Product model:TriSep®3000
Product overview

Ultra Efficiency : 250,000 theoretical plates/m

Ultra Speed : 4 compounds are baseline separated in less than 30 seconds

Ultra Peak Capacity : 3 times higher than HPLC

Ultra Reproducibility : Excellent reproducibility as in HPLC

  • Dual Mechanism of Separation

         In pressurized capillary electrochromatography (pCEC), the retention mechanism is based on both chromatographic partition and electrophoresis. The dual mechanism of pCEC dramatically enhances the separation selectively, compared to the stand alone HPLC or CE system.

    All in One  

         Modular design and flexible combination make it possible to run pCEC, μ-HPLC and CE on the same TriSep®-3000 system.

    Friendly in Coupling with Different Detectors

         Combined with on-column technologies, TriSep®-3000 pCEC system can be readily coupled with UV, LIF, EC, MS and other detectors. The pCEC technology is widely applied in various fields, including pharmaceutical, biotech, food and beverage, environmental applications.

    Ultra Performance created by dual separation mechanism and small particles of 1.5 μm. 


          Pressurized capillary electrochromatography (pCEC) is based on dualmechanism of both chromatoghraphic partition and electrophoresis, chromatography particles in the capillary column and force given by electro-osmotic flow (EOF) and pressure. Contributed to the dual mechanism, pCEC makes the separation of charged and non-charged components possible.

          With the existence of EOF, pCEC offers much better efficiency than that in HPLC.


          Chromatography theory states that increasing efficiency will increase resolution. pCEC easily makes the application of small particles of 1.5μm possible, because EOF in pCEC does not produce back pressure as in HPLC system with small particles. For the same reason, pCEC system makes the application of longer column over 500 mm possible, which also increases the efficiency of separation.

  • solvent delivery system

    Range1 μL/min-10 mL/min
    Precision0.06% RSD
    Pressure range1.0-40 MPa

    UV/vis detector 

    Light sourcedeuterium lamp/tungsten lamp
    Wavelength range190 ~ 700 nm
    Spectral bandwidth8 nm
    Wavelength accuracy±1 nm
    Wavelength reproducibility±0.1 nm
    Drift1×10-4 AU/h
    Noise level±0.25×10-5 AU
    Range0.0001 ~ 2.56 AUFS
    Light source life2000 h
    Operating temperature range4°C ~ 35°C
    Dimensions260(W) 140(H) 430(D) mm 

    High voltage power

    Voltage range0~±30 kV
    Current range0~100 μA
    Maximum output power4 W
    Weight12 kg

    Working environment

    working temperature10℃—40℃
    relative humidity≤75%
    power supply220V/110V±10%
    power frequency50/60Hz±5%

  • Pressurized capillary electrochromatography coupled with mass spectrometry(pCEC-MS)

          Figure 1. pCEC-LIF Analysis of FITC-labeled Aliphatic Amines

      Column: EP-100-20-3-C18

      Mobile phase: 50%(v/v) ACN/50mM NH4Ac(pH 3.0)

      Pressure: 1000 psi

      Power voltage: 10 kV

      Sample volume: 20 nL

      Detector: MS, sheath flow, 3 μL/min 50% (v/v) CH3OH/0.5% CH3COOH; Sheath gas flow, 12 unit/min; ESI, 4.5 kV; Scan speed: 200-2000u. 

      Sample: a) Insulin modified;  b) Insulin

    (Ref. “Pressurized Electrochromatography Coupled with Electrospray Ionization Mass Spectrometry for Analysis of Peptides and Proteins”, Anal. Chem., 2004; 76,6935-6940. Zhen Liang, Jicheng Duan, Lihua Zhang, Yukui Zhang, Chao Yan.)

    Pressurized capillary electrochromatography coupled with laser induced fluorescence detector (pCEC-LIF)

           Figure 2. pCEC-LIF Analysis of FITC-labeled Aliphatic Amines

      Column: EP-100-20-45-3-C18

      Mobile phase:

      A:10%(v/v) ACN + 10%40 mM PBS+H20

      B:65%(v/v) ACN + 10%40 mM PBS+H20

      Gradient: 0 – 30 min 100% A – 0%A

      Pressure: 1500 psi

      Power voltage:5 kV

      Detector: LIF detector

      Excitation: 473 nm

      Emission: 520 nm

      Sample: Aliphatic Amines C1 – C14 (peak 1to peak 14)



                  Figure 3. Separation of 6 opiate compounds in CEC

       Column: EP-75-15-1.5-ODS

       Mobile phase: 10 mM TRIS,5mMSDS/20% CH3CN

       Power voltage: 20kV

       Sample volume: 20 nL

       UV: 215 nm

    (Ref: “Separation ofrelated opiate compounds using capillary electrochromatography”,Electrophoresis, 21(4), 737, 2000, Lim,Jong-Tae; Zare,Richard N.;Bailey,Christopher G.; Rakestraw,David J.; Yan, Chao.)