A new type of liquid-phase micro-separation technology

Capillary electrophoresis (CE), also known as high performance capillary electrophoresis (HPCE, High Performance Capillary Electrophoresis), is a new type of liquid-phase micro-separation technology that uses elastic quartz capillary as the separation channel, DC high-voltage electric field as the driving force for the separation, and differences in the characteristics (molecular size, charge-to-mass ratio, dispersion coefficient, etc.) of the components to be measured as the basis for the separation. High Performance Capillary Electrophoresis (HPCE) is a new type of liquid-phase micro-separation technology that uses a flexible quartz capillary as the separation channel, a DC high-voltage electric field as the separation driving force, and the difference of the measured component's various characteristics (molecular size, charge-to-mass ratio, dispersion coefficient, etc.) as the basis for separation. Capillary electrophoresis can be coupled with a variety of assays.

 Laser Induced Fluorescence Detector (LIF) has a very high detection sensitivity, the lowest detection limit can reach 1×10-12M, which is 10,000 times higher than that of ordinary ultraviolet detector, and it is very suitable for the determination of trace biological samples.

Amino acids are organic compounds containing one or more basic amino groups and acidic carboxyl groups, which are the basic building blocks of biologically functional macromolecular proteins, and constitute the basic substances that make up the proteins required for animal nutrition. The high polarity, low volatility and absence of strong chromophore groups of most amino acids make their separation and detection difficult. Therefore, we established a method of derivatizing amino acids based on fluorescein isothiocyanate (FITC) using capillary electrophoresis coupled with a laser-induced fluorescence detector, and separated and detected them, which enriched the amino acid separation and detection methods.